|
Bioss
rabbit anti mouse tgf βr2  Rabbit Anti Mouse Tgf βr2, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/pmc09972623-84-13-18?v=Bioss Average 94 stars, based on 1 article reviews
rabbit anti mouse tgf βr2 - by Bioz Stars,
2026-07
94/100 stars
|
Buy from Supplier |
|
Jackson Laboratory
tgf βr2 mice  Tgf βr2 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/pmc13143635-30-25-30?v=Jackson+Laboratory Average 86 stars, based on 1 article reviews
tgf βr2 mice - by Bioz Stars,
2026-07
86/100 stars
|
Buy from Supplier |
|
Proteintech
affinity mouse tgf βr2 66636 1 ig ab 2881995 Affinity Mouse Tgf βr2 66636 1 Ig Ab 2881995, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/pmc11635228__jtd___16___11___7458___supplementary-0-79-85?v=Proteintech Average 94 stars, based on 1 article reviews
affinity mouse tgf βr2 66636 1 ig ab 2881995 - by Bioz Stars,
2026-07
94/100 stars
|
Buy from Supplier |
|
Bioss
rabbit anti mouse p tgf βr2  Rabbit Anti Mouse P Tgf βr2, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/pmc10318691-100-14-18?v=Bioss Average 95 stars, based on 1 article reviews
rabbit anti mouse p tgf βr2 - by Bioz Stars,
2026-07
95/100 stars
|
Buy from Supplier |
|
R&D Systems
anti mouse tgf βr2 Anti Mouse Tgf βr2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/pmc10232007-223-20-23?v=R%26D+Systems Average 93 stars, based on 1 article reviews
anti mouse tgf βr2 - by Bioz Stars,
2026-07
93/100 stars
|
Buy from Supplier |
|
Jackson Laboratory
mck cre mice Mck Cre Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/pmc13143635-30-18-20?v=Jackson+Laboratory Average 86 stars, based on 1 article reviews
mck cre mice - by Bioz Stars,
2026-07
86/100 stars
|
Buy from Supplier |
|
Bio-Techne corporation
recombinant mouse wnt-3a protein Recombinant Mouse Wnt 3a Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/bio-techne+corporation___1324-wn?v=Bio-Techne+corporation Average 96 stars, based on 1 article reviews
recombinant mouse wnt-3a protein - by Bioz Stars,
2026-07
96/100 stars
|
Buy from Supplier |
|
Bio-Techne corporation
recombinant mouse tgf-beta rii/(mouse) fc chimera, cf Recombinant Mouse Tgf Beta Rii/(Mouse) Fc Chimera, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/bio-techne+corporation___1600-r2?v=Bio-Techne+corporation Average 92 stars, based on 1 article reviews
recombinant mouse tgf-beta rii/(mouse) fc chimera, cf - by Bioz Stars,
2026-07
92/100 stars
|
Buy from Supplier |
|
Bio-Techne corporation
mouse igg2a isotype control Mouse Igg2a Isotype Control, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/bio-techne+corporation___mab003?v=Bio-Techne+corporation Average 94 stars, based on 1 article reviews
mouse igg2a isotype control - by Bioz Stars,
2026-07
94/100 stars
|
Buy from Supplier |
|
Bio-Techne corporation
recombinant mouse wnt-3a protein, cf Recombinant Mouse Wnt 3a Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/bio-techne+corporation___1324-wn-cf?v=Bio-Techne+corporation Average 94 stars, based on 1 article reviews
recombinant mouse wnt-3a protein, cf - by Bioz Stars,
2026-07
94/100 stars
|
Buy from Supplier |
|
Aviva Systems
antibodies against mouse dbp Antibodies Against Mouse Dbp, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/pmc04777830-327-6-10?v=Aviva+Systems Average 85 stars, based on 1 article reviews
antibodies against mouse dbp - by Bioz Stars,
2026-07
85/100 stars
|
Buy from Supplier |
|
Developmental Studies Hybridoma Bank
mouse antisarcomeric myosin heavy chain Mouse Antisarcomeric Myosin Heavy Chain, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/tgf+%CE%B2r2+mice/pm25446186-119-20-27?v=Developmental+Studies+Hybridoma+Bank Average 95 stars, based on 1 article reviews
mouse antisarcomeric myosin heavy chain - by Bioz Stars,
2026-07
95/100 stars
|
Buy from Supplier |
Image Search Results
Journal: Cellular & Molecular Biology Letters
Article Title: IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties
doi: 10.1186/s11658-023-00429-w
Figure Lengend Snippet: Primer sequences used for PCR
Article Snippet: The following primary antibodies were used: rabbit anti-mouse TGF-β2 (1: 500, Bioss, bs-20412R),
Techniques:
Journal: Cellular & Molecular Biology Letters
Article Title: IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties
doi: 10.1186/s11658-023-00429-w
Figure Lengend Snippet: The expression of TGF-β2 and TGF-βr2 in CTX-injured TA muscle. A RNA levels of TGF-βr2 and TGF-β2 in WT mice injured TA muscle, analyzed by microarray experiment and qPCR. B The protein expression of TGF-βr2 and TGF-β2 in WT mice injured TA muscle, analyzed by Western blot. The relative protein levels are expressed as a ratio (protein of interest/GAPDH). All data are presented as means ± SD ( n = 3). A one-way ANOVA was used for multiple comparisons (* P < 0.05; ** P < 0.01)
Article Snippet: The following primary antibodies were used: rabbit anti-mouse TGF-β2 (1: 500, Bioss, bs-20412R),
Techniques: Expressing, Microarray, Western Blot
Journal: Cellular & Molecular Biology Letters
Article Title: IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties
doi: 10.1186/s11658-023-00429-w
Figure Lengend Snippet: Intrinsic TGF-β signal controls immunological molecules and myokine expression in cultured primary myotubes. A Representative immunofluorescence staining for desmin, myogenin, eMHC, and TGF-βr2 in SM TGF-βr2 −/− mice-derived MPC-myotubes (MPCs) that received pro-inflammatory stimuli or not. B , C Western blot analysis of the protein expression of p-Smad2/3, H-2K b , H2-Eα, and TLR3 in MPC-myotubes that received pro-inflammatory stimuli or not. D Luminex assay of protein level changes for pro-inflammatory myokines in control or SM TGF-βr2 −/− mice-derived MPC-myotubes exposed to inflammatory milieu or not. Before creating the heat map, log transformation was used to process the data and plot in R language. The relative protein levels are expressed as a ratio (protein of interest/GAPDH). All data are presented as means ± SD ( n = 3 replicates). One-way ANOVA was used for multiple comparisons (** P < 0.01; *** P < 0.001). Bar = 50 μm
Article Snippet: The following primary antibodies were used: rabbit anti-mouse TGF-β2 (1: 500, Bioss, bs-20412R),
Techniques: Expressing, Cell Culture, Immunofluorescence, Staining, Derivative Assay, Western Blot, Luminex, Control, Transformation Assay
![Intrinsic TGF-β signaling effects on the activities of IRE1α and eIF2α arms of UPR in muscle cell. A The protein levels of IRE1α and eIF2α in damaged TA muscle from control or SM TGF-βr2 −/− mice were analyzed by Western blot. The relative protein expression values are expressed as a ratio [phosphorylated (P) protein/total (T) protein]. B Immunofluorescence staining and the quantitative results of IRE1α and eIF2α in damaged TA muscle. All data are presented as means ± SD ( n = 3). A one-way ANOVA was used for multiple comparisons (* P < 0.05; ** P < 0.01). Bar = 50 μm](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2623/pmc09972623/pmc09972623__11658_2023_429_Fig6_HTML.jpg)
Journal: Cellular & Molecular Biology Letters
Article Title: IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties
doi: 10.1186/s11658-023-00429-w
Figure Lengend Snippet: Intrinsic TGF-β signaling effects on the activities of IRE1α and eIF2α arms of UPR in muscle cell. A The protein levels of IRE1α and eIF2α in damaged TA muscle from control or SM TGF-βr2 −/− mice were analyzed by Western blot. The relative protein expression values are expressed as a ratio [phosphorylated (P) protein/total (T) protein]. B Immunofluorescence staining and the quantitative results of IRE1α and eIF2α in damaged TA muscle. All data are presented as means ± SD ( n = 3). A one-way ANOVA was used for multiple comparisons (* P < 0.05; ** P < 0.01). Bar = 50 μm
Article Snippet: The following primary antibodies were used: rabbit anti-mouse TGF-β2 (1: 500, Bioss, bs-20412R),
Techniques: Control, Western Blot, Expressing, Immunofluorescence, Staining
![Intrinsic TGF-β signaling affects muscle cell immune behaviors by prompting UPR activity under pro-inflammatory conditions. The protein levels of IRE1α and eIF2α ( A ), H-2K b , H2-Eα, and TLR3 ( B ) in control or SM TGF-βr2 −/− mice-derived MPC-myotubes, with or without stimulation of IFN-γ/LPS, Tg, or TM, were analyzed by Western blot. The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1β, IL-6, and MCP-1 in MPC-myotubes with or without 48 h stimulation of IFN-γ/LPS, Tg, or TM, analyzed by qPCR. All data are presented as means ± SD ( n = 3). A one-way ANOVA was used for multiple comparisons (* P < 0.05; ** P < 0.01)](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2623/pmc09972623/pmc09972623__11658_2023_429_Fig7_HTML.jpg)
Journal: Cellular & Molecular Biology Letters
Article Title: IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties
doi: 10.1186/s11658-023-00429-w
Figure Lengend Snippet: Intrinsic TGF-β signaling affects muscle cell immune behaviors by prompting UPR activity under pro-inflammatory conditions. The protein levels of IRE1α and eIF2α ( A ), H-2K b , H2-Eα, and TLR3 ( B ) in control or SM TGF-βr2 −/− mice-derived MPC-myotubes, with or without stimulation of IFN-γ/LPS, Tg, or TM, were analyzed by Western blot. The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1β, IL-6, and MCP-1 in MPC-myotubes with or without 48 h stimulation of IFN-γ/LPS, Tg, or TM, analyzed by qPCR. All data are presented as means ± SD ( n = 3). A one-way ANOVA was used for multiple comparisons (* P < 0.05; ** P < 0.01)
Article Snippet: The following primary antibodies were used: rabbit anti-mouse TGF-β2 (1: 500, Bioss, bs-20412R),
Techniques: Activity Assay, Control, Derivative Assay, Western Blot, Expressing
![TGF-β signaling inhibits immunological characteristics of myofibers through activating the IRE1α pathway. Western blot analyses of the expression changes of Smad pathway proteins, IRE1α and PERK pathway proteins, and immunological molecules in MPC-myotubes isolated from SM TGF-βr2 −/− mice, with or without 48 h stimulation of IFN-γ/LPS and/or SRI ( A ); 24 or 48 h stimulation of IFN-γ/LPS, SRI, 4-PBA, 4μ8C (IRE1α pathway inhibitor) or GSK (PERK pathway inhibitor) ( B , D ). The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1β, IL-6, and MCP-1 in MPC-myotubes isolated from SM TGF-βr2 −/− mice, with or without 48 h stimulation of IFN-γ/LPS, SRI and/or 4-PBA,analyzed by qPCR. All data are presented as means ± SD ( n = 3). A one-way ANOVA was used for multiple comparisons (* P < 0.05; ** P < 0.01)](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2623/pmc09972623/pmc09972623__11658_2023_429_Fig8_HTML.jpg)
Journal: Cellular & Molecular Biology Letters
Article Title: IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties
doi: 10.1186/s11658-023-00429-w
Figure Lengend Snippet: TGF-β signaling inhibits immunological characteristics of myofibers through activating the IRE1α pathway. Western blot analyses of the expression changes of Smad pathway proteins, IRE1α and PERK pathway proteins, and immunological molecules in MPC-myotubes isolated from SM TGF-βr2 −/− mice, with or without 48 h stimulation of IFN-γ/LPS and/or SRI ( A ); 24 or 48 h stimulation of IFN-γ/LPS, SRI, 4-PBA, 4μ8C (IRE1α pathway inhibitor) or GSK (PERK pathway inhibitor) ( B , D ). The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1β, IL-6, and MCP-1 in MPC-myotubes isolated from SM TGF-βr2 −/− mice, with or without 48 h stimulation of IFN-γ/LPS, SRI and/or 4-PBA,analyzed by qPCR. All data are presented as means ± SD ( n = 3). A one-way ANOVA was used for multiple comparisons (* P < 0.05; ** P < 0.01)
Article Snippet: The following primary antibodies were used: rabbit anti-mouse TGF-β2 (1: 500, Bioss, bs-20412R),
Techniques: Western Blot, Expressing, Isolation
![p-38 MAPk activity relates to TGF-β-IRE1α signaling in inflamed muscle cells. A The protein levels of Erk, Jnk, p38, and p65 in control or SM TGF-βr2 −/− mice-derived MPC-myotubes, with or without stimulation of IFN-γ/LPS, SRI, 4-PBA, and/or 4μ8C, analyzed by Western blot. The relative protein expression values are expressed as a ratio [phosphorylated (P) protein/total (T) protein]. B Immunofluorescence staining and the quantitative results of P-p38 MAPK in damaged TA muscle. All data are presented as means ± SD ( n = 3). A one-way ANOVA was used for multiple comparisons (* P < 0.05; ** P < 0.01). Bar = 50 μm](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2623/pmc09972623/pmc09972623__11658_2023_429_Fig9_HTML.jpg)
Journal: Cellular & Molecular Biology Letters
Article Title: IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties
doi: 10.1186/s11658-023-00429-w
Figure Lengend Snippet: p-38 MAPk activity relates to TGF-β-IRE1α signaling in inflamed muscle cells. A The protein levels of Erk, Jnk, p38, and p65 in control or SM TGF-βr2 −/− mice-derived MPC-myotubes, with or without stimulation of IFN-γ/LPS, SRI, 4-PBA, and/or 4μ8C, analyzed by Western blot. The relative protein expression values are expressed as a ratio [phosphorylated (P) protein/total (T) protein]. B Immunofluorescence staining and the quantitative results of P-p38 MAPK in damaged TA muscle. All data are presented as means ± SD ( n = 3). A one-way ANOVA was used for multiple comparisons (* P < 0.05; ** P < 0.01). Bar = 50 μm
Article Snippet: The following primary antibodies were used: rabbit anti-mouse TGF-β2 (1: 500, Bioss, bs-20412R),
Techniques: Activity Assay, Control, Derivative Assay, Western Blot, Expressing, Immunofluorescence, Staining
Journal: Cellular & Molecular Biology Letters
Article Title: IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties
doi: 10.1186/s11658-023-00429-w
Figure Lengend Snippet: TGF-β-IRE1α signaling inhibits immunological characteristics of muscle cells by attenuating p38 MAPK pathway. Western blot analyses of the expression changes of H-2K b , H2-Eα, or TLR3 in MPC-myotubes derived from Con and SM TGF-βr2 −/− mice ( A ), or in TGF-βr2 −/− MPC-myotubes ( B ) with or without stimulation of IFN-γ/LPS, SRI, 4μ8C, and/or SB (p38 pathway inhibitor). The relative protein expression values are expressed as a ratio (protein of interest/GAPDH). All data are presented as means ± SD ( n = 3). A one-way ANOVA was used for multiple comparisons (* P < 0.05; ** P < 0.01)
Article Snippet: The following primary antibodies were used: rabbit anti-mouse TGF-β2 (1: 500, Bioss, bs-20412R),
Techniques: Western Blot, Expressing, Derivative Assay
Journal: Frontiers in Immunology
Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle
doi: 10.3389/fimmu.2026.1810106
Figure Lengend Snippet: Muscle-specific TGF-β signaling regulates macrophage phenotypes and efferocytosis in inflamed muscle. (A) FACS analysis of the proportion of M1 (F4/80 + Ly6C + ) and M2 (F4/80 + CD206 + ) macrophages. (B) qRT-PCR analysis of gene levels of anti-inflammatory (Arg-1, Mrc1, Retlna) and pro-inflammatory (iNOS, TNF-α, IL-6) molecules in macrophages sorted from damaged muscle of control and SM TGF-βr2 -/- mice on day 3 post-myoinjury( n = 3). (C) Immunofluorescence analysis of the efferocytic macrophages (Tunel + F4/80 + ) and the free macrophages (Tunel - F4/80 + ). White arrow indicates the efferocytic macrophages. (D) FACS analysis of the proportion of F4/80 + Tunel + , and F4/80 + Tunel - macrophages. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.
Article Snippet: Skeletal muscle-specific TGF-β receptor 2 knockout mice (referred to as SM TGF-βr2 −/− ) were generated by crossing MCK-Cre mice (The Jackson Laboratory) with floxed
Techniques: Quantitative RT-PCR, Control, Immunofluorescence, TUNEL Assay
Journal: Frontiers in Immunology
Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle
doi: 10.3389/fimmu.2026.1810106
Figure Lengend Snippet: Muscle TGF-β signaling prompts to Tregs-mediated macrophage efferocytosis by suppressing myofiber IL-6 production. (A) Western blot and immunofluorescence analysis show IL-6 and p-STAT3 expression in inflamed muscle ( n = 3). (B) Western blot analysis of IL-6 and p-STAT3 protein levels in control or SM TGF-βr2 -/- MPC-myotubes cultured in pro-inflammatory milieu, with or without SRI administration ( n = 3). (C) FACS analysis of the proportion of CD25 + p-STAT3 + cells and CD25 + gp130 + cells in inflamed muscle ( n = 4). (D) FACS analysis of the proportion of CD25 + Foxp3 + cells and CD25 + IL-13 + cells in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). (E) qRT-PCR analysis of Foxp3 and IL-13 mRNA levels in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.
Article Snippet: Skeletal muscle-specific TGF-β receptor 2 knockout mice (referred to as SM TGF-βr2 −/− ) were generated by crossing MCK-Cre mice (The Jackson Laboratory) with floxed
Techniques: Western Blot, Immunofluorescence, Expressing, Control, Cell Culture, Quantitative RT-PCR
Journal: Frontiers in Immunology
Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle
doi: 10.3389/fimmu.2026.1810106
Figure Lengend Snippet: Myofiber TGF-β signaling regulates Tregs-mediated macrophage efferocytosis in vitro. (A) Scheme of the co-culture experiment. (B) Fluorescence staining shows DiD + ACs engulf by macrophages in co-culture system with or without TGF-βr2 -/- MPC-myotubes, SRI, or AH. (C) FACS analysis of the proportion of F4/80 + CD206 + M2 macrophages and macrophages engulfed DiD + ACs in co-culture system. (D) FACS analysis of the proportion of F4/80 + IL-10 + , F4/80 + p-STAT3 + , F4/80 + Vav1 + and F4/80 + Rac1 + cells. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm.
Article Snippet: Skeletal muscle-specific TGF-β receptor 2 knockout mice (referred to as SM TGF-βr2 −/− ) were generated by crossing MCK-Cre mice (The Jackson Laboratory) with floxed
Techniques: In Vitro, Co-Culture Assay, Fluorescence, Staining
Journal: Cell Communication and Signaling : CCS
Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling
doi: 10.1186/s12964-023-01163-8
Figure Lengend Snippet: Primer sequences used for phenotype identification of SM TGF-βr2 −/− mice
Article Snippet: The following protein antibodies were labeled: rabbit anti-mouse TGF-βr2 or TGF-β2 (1:500, eBioscience, USA),
Techniques:
Journal: Cell Communication and Signaling : CCS
Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling
doi: 10.1186/s12964-023-01163-8
Figure Lengend Snippet: Primer sequences used for PCR
Article Snippet: The following protein antibodies were labeled: rabbit anti-mouse TGF-βr2 or TGF-β2 (1:500, eBioscience, USA),
Techniques:
Journal: Cell Communication and Signaling : CCS
Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling
doi: 10.1186/s12964-023-01163-8
Figure Lengend Snippet: The inflammatory stimulus drives the myofiber specific TGF-β signal activation. A , RNA-seq analysis showing the mRNA level changes of TGF-β2 and TGF-βr2 of inflamed TA muscle. B , The mRNA levels of TGF-β2 and TGF-βr2 of inflamed muscle were quantified by qRT-PCR. C , Western blot analysis of protein levels in TGF-β2, p-TGF-βr2 and p-Smad2/3 in inflamed muscle. The relative protein level values were expressed as a ratio (protein of interest/GAPDH or phosphorylated (p) protein/total protein). D , Immunofluorescence double-staining results of TGF-βr2, p-TGF-βr2, p-Smad2/3 and Dystrophin in inflamed muscle. E , Western blot analysis of the protein levels of TGF-β2, p-TGF-βr2 and p-Smad2/3 in MPC-Myotubes receiving pro-inflammatory administration. Statistical data were expressed as the mean ± SD ( n = 3). Multiple comparisons were analyzed by One-way ANOVA (* P < 0.05, ** P < 0.01). Bar = 50 μm
Article Snippet: The following protein antibodies were labeled: rabbit anti-mouse TGF-βr2 or TGF-β2 (1:500, eBioscience, USA),
Techniques: Activation Assay, RNA Sequencing Assay, Quantitative RT-PCR, Western Blot, Immunofluorescence, Double Staining
Journal: Cell Communication and Signaling : CCS
Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling
doi: 10.1186/s12964-023-01163-8
Figure Lengend Snippet: Endogenous TGF-β signaling regulates IL-10 production in regenerating myofiber. Transcriptome assay ( A ), qRT-PCR analysis ( B ) and Luminex analysis ( C ) of gene levels of some myokines, mRNA level of IL-10, protein levels of some myokines in TGF-βr2 −/− - or control-MPC-Myotubes exposed to pro-inflammatory milieu, respectively. qRT-PCR analysis ( D ) of IL-10 mRNA level in inflamed muscle. Immunofluorescence staining ( E ) showed IL-10 expression change in regenerating myofibers. Multiple comparisons were analyzed by One-way ANOVA. Statistical data were expressed as mean ± SD ( n = 3). (* P < 0.05, ** P < 0.01). Bar = 50 μm
Article Snippet: The following protein antibodies were labeled: rabbit anti-mouse TGF-βr2 or TGF-β2 (1:500, eBioscience, USA),
Techniques: Quantitative RT-PCR, Luminex, Control, Immunofluorescence, Staining, Expressing
Journal: Cell Communication and Signaling : CCS
Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling
doi: 10.1186/s12964-023-01163-8
Figure Lengend Snippet: Myofiber TGF-β-IL-10 signaling effects on macrophages efferocytosis. Immunofluorescence staining (upper part) and FACS analysis (lower part) of the uptake of PKH67 labeled apoptotic cells by macrophage co-cultured with TGF-βr2 −/− - or control-MPC-Myotubes exposed to pro-inflammatory milieu, and treated with or without SRI, rmIL-10 or AS101. Mac: Macrophage; ACs: Apoptotic cells; Multiple comparisons were analyzed by One-way ANOVA. Statistical data were expressed as mean ± SD ( n = 3). (* P < 0.05, ** P < 0.01). Bar = 50 μm
Article Snippet: The following protein antibodies were labeled: rabbit anti-mouse TGF-βr2 or TGF-β2 (1:500, eBioscience, USA),
Techniques: Immunofluorescence, Staining, Labeling, Cell Culture, Control
Journal: Cell Communication and Signaling : CCS
Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling
doi: 10.1186/s12964-023-01163-8
Figure Lengend Snippet: Myofiber TGF-β-IL-10 signaling effects on macrophage phenotype and efferocytosis related-molecules expression. FACS analysis of Bcl3, p-STAT3, Vav1, GTPase Rac1, CD206 and CX3CR1 in macrophages co-cultured with TGF-βr2. −/− - or control-MPC-Myotubes, exposed to pro-inflammatory milieu, and treated with or without SRI, rmIL-10 or AS101, respectively. Multiple comparisons were analyzed by One-way ANOVA. Statistical data were expressed as mean ± SD ( n = 3). (* P < 0.05, ** P < 0.01)
Article Snippet: The following protein antibodies were labeled: rabbit anti-mouse TGF-βr2 or TGF-β2 (1:500, eBioscience, USA),
Techniques: Expressing, Cell Culture, Control